Bio 3 Kawashima Clearance Requirement

Please see the following list for your choice of clearance requirement.
Email me at str2kawashima@gmail.com to apply for a topic. A maximum of 2 people may register for each number.

1. Gene cloning
2. Dideoxy chain termination/Sanger method for nucleotide sequencing
3. Shotgun genome sequencing
   
4. Polymerase chain reaction
5. Restriction fragment length polymorphism
6. Southern blot
7. Replication
8. Telomeres and telomerases
   
9. Transcription
10. Translation
11. Operon and negative feedback loop
    
12. Gene expression
13. Restriction enzyme digestion
14. Griffith experiment
15. Hershey and Chase experiment
    
16. Messelson and Stahl experiment
17. Avery, MacLeod and McCarty experiment
18. Human Genome Project
19. Plant genetic engineering methods (choose one)
    - Agrobacterium
    - Gene gun
    - Protoplast chemical transformation
20. Animal genetic engineering methods (choose one)
    - Viral transfection
    - Liposmes and lipid carriers
    - Others

DNA Technology and Tools Animations

Transcription and Translation

Transcription and Translation

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Translation


Agarose Gel Electrophoresis


Polymerase Chain Reaction


Human Genome Project


Chain termination method for nucleotide sequencing (Sanger Method)


Gene Cloning and Recombinant DNA


DNA detection by Southern Blot


cDNA


Sources:
Sadava, et al., Life: The Science of Biology, Eighth Edition, Sinauer Associates

Alberts, et al., Molecular Biology of the Cell, Fifth Edition, Garland Science Publishing

Prescott, et al., Microbiology, Sixth Edition, McGraw-Hill Higher Education

DNA Fingerprinting Activity

Please read the "Instructions" document first. Restriction enzyme choice is according to your Bio groupings -
BamH1 (Salamat, San Juan, Malubag, Lasco, Panton, Nadura)
Pst1 (Pangilinan, Tobillo, Ylanan, Garcia, Tamayo, Tarcelo)
Sma1(Valdez, Gallardo, Lopez, Manalo, Rahayel, Rodelas)
Xma1 (Estanislao, Gomez, Alegre, Santos, Galido, Co)
BsrF1 (Lim, Asis, Estrada, Rodriguez, Evangelista, Alba)

INDIVIDUAL. Due on Bio perio day :>

Activity DNA Fingerprinting Instructions

Activity DNA Fingerprinting Restriction Enzymes

100 bp ladder

Transcription and Translation

Transcription and Translation

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FINDTHEHIDDENMESSAGE

Telomeres and Telomerase Activity

DNA Replication